Peptide desalting
AttractSPE®Tips are SPE tips, compatible with automates for high
throughput screening, useful for biomolecular applications of peptide desalting,
proteomics and biomarker discovery.
About
Process of use: centrifugation, positive pressure.
Our SPE tips can’t be used with a pipette.
Formats: 10µL, 200µL and 1mL.
For any other format, contact us.
NEW: eco packaging of 960 tips in bulk now available for AttractSPE® Tips C18!
- More economical: the bulk packaging represents a saving of up to 30% compared to the price of 10 boxes of 96 tips!
- More ecological: new packaging is made with 90% less plastic and is 100% recyclable!
Contact us for more details!
Capacity
Each product is available with different capacities, which are designated T1, T2 or T3 in the product designations. This designation refers to the layer thickness of SPE tips. For a more reliable product, each tool contains only ONE layer.
Table legend: T1, T2, T3 = maximum loading capacity (µg)
Details regarding the loading and elution volumes for our different BioSPE® PurePep products to find the best product depending on your sample volume.
Information table regarding the capacities (maximal amount of peptides/proteins that can be retained) of our different BioSPE®PurePep products :
- For Standard capacity, product references contain the letter S (e.g. Tips-PurePep.S.200.96),
- For High capacity, product references contain the letter H (e.g. Tips-PurePep.H.200.96).
Literature
Hijazi, H. et al. (2025) ‘Mind Your Spectra: Points to be Aware of When Validating the Identification of Isobaric Histone Peptidoforms’, Journal of Proteome Research.
In their latest publication, Hassan M. Hijazi et al. investigate the identification of mouse-specific histone variants H3mm7 and H3mm13 from brain and testis extracts using LC-MS/MS.
To enable accurate detection of post-translational modifications such as acetylation and lactylation, peptide purification was performed with Affinisep BioSPE® PurePep, which proved highly effective in removing interfering substances and preserving modified peptides.
This approach improved MS/MS spectral quality, particularly for critical ions like b1 and CycIm, and helped address challenges related to isobaric variants caused by uncontrolled modifications such as propionylation.
Product used : BioSPE® PurePep Tips 200µl (Catalog # KT-Adapt-Tips-PurePep.H.200.96)
Application notes